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gpr [2019-07-05 11:32:17]
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gpr [2019-07-05 11:32:17]

germination protease (degradation of SASPs)
locus
BSU25540
pI
5.20
mw
40.12 kDa
protein length
368 aa Sequence Blast
gene length
1107 bp Sequence Blast
function
degradation of SASPs
product
germination protease (degradation of SASPs)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.3|Proteolysis during sporulation/ germination]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.4|Germination] → [category|SW 4.2.4.2|Additional germination proteins]

    • Gene

    Coordinates
    2,634,505 2,635,611

    The protein

    Catalyzed reaction/ biological activity

  • degradation of small, acid-soluble spore proteins (SASPs) [Pubmed|8478323]

    • Protein family

  • peptidase A25 family (according to Swiss-Prot)

    • Structure

  • [PDB|1C8B] (from B. megaterium, 68% identity) [pubmed|10864493]

    • [SW|Localization]

  • inner spore membrane [Pubmed|26731423]

    • Expression and Regulation

    Operons

    genes
    [gene|91C5AF928EB8E00F12D1BC0E788E1CB116F87B04|gpr]-[gene|C9817A54C8E72193280E393D7BD3375BD1751738|spoIIP]
    description
    [Pubmed|16497325]

    sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|16497325,1840582], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|1840582], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

    • regulatory mechanism

  • [protein|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|SpoVT]: repression, [Pubmed|16497325], in [regulon|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|SpoVT regulon]

    • regulation

  • expressed during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF], [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG], [SW|SpoVT]) [Pubmed|16497325,1840582]
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    Biological materials

    Mutant

  • BKE25540 ([gene|91C5AF928EB8E00F12D1BC0E788E1CB116F87B04|gpr]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACAGGGGCCTCCTTCAC, downstream forward: _UP4_TAAACAGTTCTACTTCCTCT
  • BKK25540 ([gene|91C5AF928EB8E00F12D1BC0E788E1CB116F87B04|gpr]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACAGGGGCCTCCTTCAC, downstream forward: _UP4_TAAACAGTTCTACTTCCTCT

    • References

  • 16199582,9045848,8188581,8071242,1732215,16497325,7961468,8071212,9748439,1840582,7608092,8478323,23927687,26731423,10864493